EphA2/Ephrin-A1 signaling complexes restrict corneal epithelial cell migration.

ABSTRACT:

Purpose

Eph/ephrin signaling proteins are present in the corneal epithelium, where their function remains unknown. The authors examined the role of the EphA2 receptor and ephrin-A1 ligand in human corneal epithelial cell migration.

Methods

Immunohistochemical analysis of EphA2 and ephrin-A1 in healthy and diabetic corneas was performed in concert with linear scratch wound healing studies in primary and telomerase-immortalized human corneal epithelial cells. Corneal epithelial cells were exposed to a soluble ephrin-A1-Fc peptide mimetic that targets EphA2 to trigger receptor phosphorylation and subsequent downregulation. Genetic modulation of EphA2 and ephrin-A1 levels was combined with manipulation of Erk1/2 or Akt signaling during wound healing.

Results

EphA2 was imm

SUBMITTER: Kaplan N 

PROVIDER: S-EPMC3317430 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature