A comparative study on the expression, purification and functional characterization of human adiponectin in Pichia pastoris and Escherichia coli.

ABSTRACT: Adiponectin is one of the most bioactive substances secreted by adipose tissue and is involved in the protection against metabolic syndrome, artherosclerosis and type II diabetes. Research into the use of adiponectin as a promising drug for metabolic syndromes requires production of this hormone in high quantities considering its molecular isoforms. The objective of this study is to produce recombinant human adiponectin by Pichia pastoris (P-ADP) as a cheap and convenient eukaryotic expression system for potential application in pharmaceutical therapy. For comparison, adiponectin was also expressed using the Escherichia coli (E-ADP) expression system. Adiponectin was constructed by overlap-extension PCR, and cloned in standard cloning vector and hosts. Recombinant expression vectors were c

SUBMITTER: Rothan HA 

PROVIDER: S-EPMC3317727 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature