Single-image diffusion coefficient measurements of proteins in free solution.

ABSTRACT: Diffusion coefficient measurements are important for many biological and material investigations, such as studies of particle dynamics and kinetics, and size determinations. Among current measurement methods, single particle tracking (SPT) offers the unique ability to simultaneously obtain location and diffusion information about a molecule while using only femtomoles of sample. However, the temporal resolution of SPT is limited to seconds for single-color-labeled samples. By directly imaging three-dimensional diffusing fluorescent proteins and studying the widths of their intensity profiles, we were able to determine the proteins' diffusion coefficients using single protein images of submillisecond exposure times. This simple method improves the temporal resolution of diffusion coefficien

SUBMITTER: Zareh SK 

PROVIDER: S-EPMC3318113 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature