Functional and genetic characterization of the tap efflux pump in Mycobacterium bovis BCG.

ABSTRACT: Efflux pumps extrude a wide variety of chemically unrelated compounds conferring multidrug resistance and participating in numerous physiological processes. Mycobacterium tuberculosis possesses many efflux pumps, and their roles in drug resistance and physiology are actively investigated. In this work we found that tap mutant cells showed changes in morphology and a progressive loss of viability upon subcultivation in liquid medium. Transcriptome analysis in Mycobacterium bovis BCG revealed that disruption of the Rv1258c gene, encoding the Tap efflux pump, led to an extensive change in gene expression patterns during stationary phase, with no changes during exponential growth. In stationary phase, Tap inactivation triggered a general stress response and led to a general repression of genes

SUBMITTER: Ramon-Garcia S 

PROVIDER: S-EPMC3318366 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature