Classically activated macrophages use stable microtubules for matrix metalloproteinase-9 (MMP-9) secretion.

ABSTRACT: As major effector cells of the innate immune response, macrophages must adeptly migrate from blood to infected tissues. Endothelial transmigration is accomplished by matrix metalloproteinase (MMP)-induced degradation of basement membrane and extracellular matrix components. The classical activation of macrophages with LPS and IFN-γ causes enhanced microtubule (MT) stabilization and secretion of MMPs. Macrophages up-regulate MMP-9 expression and secretion upon immunological challenge and require its activity for migration during the inflammatory response. However, the dynamics of MMP-9 production and intracellular distribution as well as the mechanisms responsible for its trafficking are unknown. Using immunofluorescent imaging, we localized intracellular MMP-9 to small Golgi-derived cytopl

SUBMITTER: Hanania R 

PROVIDER: S-EPMC3318683 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature