Comprehensive set of integrative plasmid vectors for copper-inducible gene expression in Myxococcus xanthus.

ABSTRACT: Myxococcus xanthus is widely used as a model system for studying gliding motility, multicellular development, and cellular differentiation. Moreover, M. xanthus is a rich source of novel secondary metabolites. The analysis of these processes has been hampered by the limited set of tools for inducible gene expression. Here we report the construction of a set of plasmid vectors to allow copper-inducible gene expression in M. xanthus. Analysis of the effect of copper on strain DK1622 revealed that copper concentrations of up to 500 μM during growth and 60 μM during development do not affect physiological processes such as cell viability, motility, or aggregation into fruiting bodies. Of the copper-responsive promoters in M. xanthus reported so far, the multicopper oxidase cuoA promoter was us

SUBMITTER: Gomez-Santos N 

PROVIDER: S-EPMC3318791 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature