Proinflammatory role of leukocyte-derived Egr-1 in the development of murine postoperative ileus.

ABSTRACT:

Background & aims

Early growth response gene-1 (Egr-1) is an important inflammatory transcription factor. We hypothesize that leukocyte-derived Egr-1 plays a key inflammatory role in causing postoperative ileus.

Methods

Wild-type, Egr-1 knockout, and chimera mice (constructed by irradiation followed by injection with Egr-1(+/+) or Egr-1(-/-) bone marrow) were subjected to surgical manipulation of the gastrointestinal tract to induce ileus. Reverse-transcription polymerase chain reaction, Western blot, and immunohistochemistry quantified and localized Egr-1. Lumenal transit of nonabsorbable fluorescein isothiocyanate-labeled dextran and in vitro organ bath techniques measured functional gastrointestinal motility. Inflammatory mediator expressions were measured by Griess react

SUBMITTER: Schmidt J 

PROVIDER: S-EPMC3319384 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature