Comparative researches on two direct transmethylation without prior extraction methods for fatty acids analysis in vegetal matrix with low fat content.

ABSTRACT:

Background

The aim of our work was to compare two methods, both based on direct transmethylation with different reagents, BF3/MeOH (boron trifluoride in methanol) or HCl/MeOH (hydrochloride acid in methanol), in acid catalysis, without prior extraction, to find the fast, non-expensive but enough precise method for 9 principal fatty acids (lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic, arahidic and behenic acids) analysis in vegetal matrix with low fat content (forage from grassland), for nutrition and agrochemical studies.

Results

Comparatively, between the average values obtained for all analysed fatty acids by the two methods based on direct transmethylation without prior extraction no significantly difference was identified (p > 0.05). The results of fat

SUBMITTER: Harmanescu M 

PROVIDER: S-EPMC3319428 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature