Determination of peptide-surface adsorption free energy for material surfaces not conducive to SPR or QCM using AFM.

ABSTRACT: The interactions between peptides and proteins with material surfaces are of primary importance in many areas of biotechnology. While surface plasmon resonance spectroscopy (SPR) and quartz crystal microbalance (QCM) methods have proven to be very useful in measuring fundamental properties characterizing adsorption behavior, such as the free energy of adsorption for peptide-surface interactions, these methods are largely restricted to use for materials that can readily form nanoscale-thick films over the respective sensor surfaces. Many materials including most polymers, ceramics, and inorganic glasses, however, are not readily suitable for use with SPR or QCM methods. To overcome these limitations, we recently showed that desorption forces (F(des)) obtained using a standardized AFM method

SUBMITTER: Thyparambil AA 

PROVIDER: S-EPMC3319500 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature