A full suite of histone and histone modifying genes are transcribed in the dinoflagellate Lingulodinium.

ABSTRACT:

Background

Dinoflagellates typically lack histones and nucleosomes are not observed in DNA spreads. However, recent studies have shown the presence of core histone mRNA sequences scattered among different dinoflagellate species. To date, the presence of all components required for manufacturing and modifying nucleosomes in a single dinoflagellate species has not been confirmed.

Methodology and results

Analysis of a Lingulodinium transcriptome obtained by Illumina sequencing of mRNA shows several different copies of each of the four core histones as well as a suite of histone modifying enzymes and histone chaperone proteins. Phylogenetic analysis shows one of each Lingulodinium histone copies belongs to the dinoflagellate clade while the second is more divergent and does not

SUBMITTER: Roy S 

PROVIDER: S-EPMC3319573 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature