Identification of new hematopoietic cell subsets with a polyclonal antibody library specific for neglected proteins.

ABSTRACT: The identification of new markers, the expression of which defines new phenotipically and functionally distinct cell subsets, is a main objective in cell biology. We have addressed the issue of identifying new cell specific markers with a reverse proteomic approach whereby approximately 1700 human open reading frames encoding proteins predicted to be transmembrane or secreted have been selected in silico for being poorly known, cloned and expressed in bacteria. These proteins have been purified and used to immunize mice with the aim of obtaining polyclonal antisera mostly specific for linear epitopes. Such a library, made of about 1600 different polyclonal antisera, has been obtained and screened by flow cytometry on cord blood derived CD34+CD45dim cells and on peripheral blood derived mat

SUBMITTER: Moro M 

PROVIDER: S-EPMC3319577 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature