Proteomic analysis yields an unexpected trans-acting point in control of the human sympathochromaffin phenotype.

ABSTRACT:

Background

The secretory protein chromogranin A (CHGA) plays a necessary role in formation of catecholamine storage vesicles and gives rise to a catecholamine release-inhibitory fragment. Because genetic variation in the proximal human CHGA promoter predicts autonomic function and blood pressure, we explored how a common genetic variant alters transcription of the gene.

Methods and results

Bioinformatic analysis suggested that the common G-462A promoter variant (rs9658634) may disrupt as many as 3 transcriptional control motifs: LEF1, COUP-TF, and PPARγ-RXRα. During electrophoretic mobility shifts, chromaffin cell nuclear proteins bound specifically to the A (though not G) allele of CHGA promoter G-462A. On oligonucleotide affinity chromatography followed by electrospray ion

SUBMITTER: Chiron S 

PROVIDER: S-EPMC3319683 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature