Molecular machines in the synapse: overlapping protein sets control distinct steps in neurosecretion.

ABSTRACT: Activity regulated neurotransmission shapes the computational properties of a neuron and involves the concerted action of many proteins. Classical, intuitive working models often assign specific proteins to specific steps in such complex cellular processes, whereas modern systems theories emphasize more integrated functions of proteins. To test how often synaptic proteins participate in multiple steps in neurotransmission we present a novel probabilistic method to analyze complex functional data from genetic perturbation studies on neuronal secretion. Our method uses a mixture of probabilistic principal component analyzers to cluster genetic perturbations on two distinct steps in synaptic secretion, vesicle priming and fusion, and accounts for the poor standardization between different stu

SUBMITTER: Cornelisse LN 

PROVIDER: S-EPMC3320570 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature