A human 3' miR-499 mutation alters cardiac mRNA targeting and function.

ABSTRACT:

Rationale

MyomiRs miR-499, miR-208a and miR-208b direct cardiac myosin gene expression. Sequence complementarity between miRs and their mRNA targets determines miR effects, but the functional consequences of human myomiR sequence variants are unknown.

Objective

To identify and investigate mutations in human myomiRs in order to better understand how and to what extent naturally-occurring sequence variation can impact miR-mRNA targeting and end-organ function.

Methods and results

Screening of ≈2,600 individual DNAs for myomiR sequence variants identified a rare mutation of miR-499, u17c in the 3' end, well outside the seed region thought to determine target recognition. In vitro luciferase reporter analysis showed that the 3' miR-499 mutation altered suppression of a su

SUBMITTER: Dorn GW 

PROVIDER: S-EPMC3320730 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature