Hepatic expression profiling in smolting and adult coho salmon (Onchorhynchus kisutch).

ABSTRACT: Coho salmon are a critical Pacific salmon species that undergo complex physiological transformations as they migrate towards seawater and enter adult life stages. During these periods, coho may receive exposure to waterborne pollutants that coincide with outmigration through contaminated waterways and return to natal streams. However, little is known regarding the ontogenic modulation of gene expression during these critical life stages. Accordingly, the purpose of the present study was to characterize the hepatic transcriptome of smolting coho, adult males, and adult females by carrying out microarray analysis with a commercially available 16,000 cDNA element platform. Quantitative PCR (Q-PCR) analysis of genes involved in chemical biotransformation (cytochrome P450 isoforms 1A, and 2M1,

SUBMITTER: Gallagher EP 

PROVIDER: S-EPMC3321376 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature