Pharmacologic effects of 2-methoxyestradiol on angiotensin type 1 receptor down-regulation in rat liver epithelial and aortic smooth muscle cells.

ABSTRACT:

Background

Delayed onset of cardiovascular disease (CVD) in female patients is not well understood, but could be due in part to the protective effect of estrogen before menopause. Experimental studies have identified the angiotensin type 1 receptor (AT1R) as a key factor in the progression of CVD.

Objective

We examined the effects of the estrogen metabolite 2-methoxyestradiol (2ME2) on AT1R expression.

Methods

Rat liver cells were exposed to 2ME2 for 24 hours, and angiotensin II (AngII) binding and AT1R mRNA expressions were assessed.

Results

In the presence of 2ME2, cells exhibited significant down-regulation of AngII binding that was both dose and time dependent, independent of estrogen receptors (ERα/ERβ). Down-regulation of AngII binding was AT1R specific,

SUBMITTER: Koganti S 

PROVIDER: S-EPMC3322289 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature