LC/LC-MS/MS of an innovative prostate human epithelial cancer (PHEC) in vitro model system.

ABSTRACT: This work describes the proteomic characterization of a novel in vitro prostate cancer model system, the clonal prostatic human epithelial cancer (PHEC) cell lines. The model is composed of three cell lines representing the three progressive cancer states found in vivo: non-tumorigenic, tumorigenic, and metastatic. The cell lines were evaluated for differential protein expression between states using two dimensional liquid:liquid chromatographic separation followed by mass spectral identification. The proteins from cellular extracts were first separated using liquid:liquid primary separation based on their isoelectric points and hydrophobicity. The resulting peptide fractions were applied to liquid chromatography-mass spectrometry (LC-MS) separation for mass determination and protein ident

SUBMITTER: Lapek JD 

PROVIDER: S-EPMC3322323 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature