Human urinary glycoproteomics; attachment site specific analysis of N- and O-linked glycosylations by CID and ECD.

ABSTRACT: Urine is a complex mixture of proteins and waste products and a challenging biological fluid for biomarker discovery. Previous proteomic studies have identified more than 2800 urinary proteins but analyses aimed at unraveling glycan structures and glycosylation sites of urinary glycoproteins are lacking. Glycoproteomic characterization remains difficult because of the complexity of glycan structures found mainly on asparagine (N-linked) or serine/threonine (O-linked) residues. We have developed a glycoproteomic approach that combines efficient purification of urinary glycoproteins/glycopeptides with complementary MS-fragmentation techniques for glycopeptide analysis. Starting from clinical sample size, we eliminated interfering urinary compounds by dialysis and concentrated the purified ur

SUBMITTER: Halim A 

PROVIDER: S-EPMC3322569 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature