Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production.

ABSTRACT: The HIV-1 Rev protein plays a key role in the late phase of virus replication. It binds to the Rev Response Element found in underspliced HIV mRNAs, and drives their nuclear export by the CRM1 receptor pathway. Moreover, mounting evidence suggests that Rev has additional functions in viral replication. Here we employed proteomics and statistical analysis to identify candidate host cell factors that interact with Rev. For this we studied Rev complexes assembled in vitro with nuclear or cytosolic extracts under conditions emulating various intracellular environments of Rev. We ranked the protein-protein interactions by combining several statistical features derived from pairwise comparison of conditions in which the abundance of the binding partners changed. As a validation set, we selected

SUBMITTER: Naji S 

PROVIDER: S-EPMC3322577 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature