Repair of the injured adult heart involves new myocytes potentially derived from resident cardiac stem cells.

ABSTRACT:

Rationale

The ability of the adult heart to generate new myocytes after injury is not established.

Objective

Our purpose was to determine whether the adult heart has the capacity to generate new myocytes after injury, and to gain insight into their source.

Methods and results

Cardiac injury was induced in the adult feline heart by infusing isoproterenol (ISO) for 10 days via minipumps, and then animals were allowed to recover for 7 or 28 days. Cardiac function was measured with echocardiography, and proliferative cells were identified by nuclear incorporation of 5-bromodeoxyuridine (BrdU; 7-day minipump infusion). BrdU was infused for 7 days before euthanasia at days 10, 17, and 38 or during injury and animals euthanized at day 38. ISO caused reduction in cardiac func

SUBMITTER: Angert D 

PROVIDER: S-EPMC3322670 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature