Detection of gastric cancer-associated microRNAs on microRNA microarray comparing pre- and post-operative plasma.

ABSTRACT:

Background

Recently, it was reported that plasma microRNAs (miRNAs) are low-invasive useful biomarkers for cancer. We attempted to isolate gastric cancer (GC)-associated miRNAs comparing pre- and post-operative paired plasma, thereby excluding the possible effects of individual variability.

Methods

This study was divided into four steps: (1) microarray analysis comparing pre- and post-operative plasma; (2) validation of candidate miRNAs by quantitative RT-PCR; (3) validation study of selected miRNAs using paired plasma; and (4) comparison of the levels of selected miRNAs in plasma between healthy controls and patients.

Results

From the results of microarray analysis, nine candidate miRNAs the levels of which were markedly decreased in post-operative plasma were select

SUBMITTER: Konishi H 

PROVIDER: S-EPMC3322946 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature