A novel procedure for absolute real-time quantification of gene expression patterns.

ABSTRACT:

Background

Temporal and tissue-specific patterns of gene expression play important roles in functionality of a biological system. Real-time quantitative polymerase chain reaction (qPCR) technique has been widely applied to single gene expressions, but its potential has not been fully released as most results have been obtained as fold changes relative to control conditions. Absolute quantification of transcripts as an alternative method has yet to gain popularity because of unresolved issues.

Results

We propose a solution here with a novel procedure, which may accurately quantify the total cDNA conventionally prepared from a biological sample at the resolution of ~70 pg/μl, and reliably estimate the absolute numbers of transcripts in a picogram of cDNA. In comparison to the

SUBMITTER: Lu Y 

PROVIDER: S-EPMC3323441 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature