Stable mutated tau441 transfected SH-SY5Y cells as screening tool for Alzheimer's disease drug candidates.

ABSTRACT: The role of hyperphosphorylation of the microtubule-associated protein tau in the pathological processes of several neurodegenerative diseases is becoming better understood. Consequently, development of new compounds capable of preventing tau hyperphosphorylation is an increasingly hot topic. For this reason, dependable in vitro and in vivo models that reflect tau hyperphosphorylation in human diseases are needed. In this study, we generated and validated an in vitro model appropriate to test potential curative and preventive compound effects on tau phosphorylation. For this purpose, a stably transfected SH-SY5Y cell line was constructed over-expressing mutant human tau441 (SH-SY5Y-TMHT441). Analyses of expression levels and tau phosphorylation status in untreated cells confirmed relevance

SUBMITTER: Loffler T 

PROVIDER: S-EPMC3323815 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature