Interactions of a platinum-modified perylene derivative with the human telomeric G-quadruplex.

ABSTRACT: The interactions of a newly synthesized platinum-modified perylene derivative, compound 7 ([{Pt(dien)}(2)(μ-4-S,S')](NO(3))(4) (dien = diethylenetriamine, 4 = N,N'-bis(1-(2-aminoethyl)-1,3-dimethylthiourea)-3,4,9,10-perylenetetracarboxylic acid diimide), with the human telomeric repeat were studied using various model oligo(deoxy)ribonucleotides to mimic the polymorphic nature of the telomeric G-quadruplex. UV/visible spectroscopy, CD spectropolarimetry, electrospray mass spectrometry (ES-MS), and isothermal titration calorimetry (ITC) were used to demonstrate that compound 7 selectively recognizes the antiparallel form of the unimolecular telomeric G-quadruplex formed by the sequence d(TTAGGG)(4) (dG-24), to which it binds with a 2:1 stoichiometry and nanomolar affinity. Compared with tel

SUBMITTER: Rao L 

PROVIDER: S-EPMC3324090 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature