High expression of p16INK4a and low expression of Bmi1 are associated with endothelial cellular senescence in the human cornea.

ABSTRACT:

Purpose

Determine cyclin-dependent kinase inhibitor 2A (p16(Ink4a)) and polycomb ring finger oncogene (Bmi1) expression in corneal endothelium samples from different age groups and test whether the expression of p16(INK4a) and Bmi1 are associated with endothelial cellular senescence in human cornea.

Methods

Samples were selected from an eyebank of healthy human corneal endothelial cells (HCECs). Donor human corneas were divided into three age-groups: age ≤30 years, 30-50 years and ≥50 years. The expression of p16(INK4a) and Bmil were analyzed by real-time PCR, immunohistochemistry, and immunofluorescence.

Results

Through real-time PCR, we detected less than threefold decreases in Bmi1 expression and greater than fivefold increases in p16(INK4a) expression associated w

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3324359 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature