Redox control of 20S proteasome gating.

ABSTRACT:

Unlabelled

The proteasome is the primary contributor in intracellular proteolysis. Oxidized or unstructured proteins can be degraded via a ubiquitin- and ATP-independent process by the free 20S proteasome (20SPT). The mechanism by which these proteins enter the catalytic chamber is not understood thus far, although the 20SPT gating conformation is considered to be an important barrier to allowing proteins free entrance. We have previously shown that S-glutathiolation of the 20SPT is a post-translational modification affecting the proteasomal activities.

Aims

The goal of this work was to investigate the mechanism that regulates 20SPT activity, which includes the identification of the Cys residues prone to S-glutathiolation.

Results

Modulation of 20SPT activity by prote

SUBMITTER: Silva GM 

PROVIDER: S-EPMC3324812 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature