Transcriptome analysis of silver carp (Hypophthalmichthys molitrix) by paired-end RNA sequencing.

ABSTRACT: The silver carp (Hypophthalmichthys molitrix) is among the most intensively pond-cultured fish species and is used in the wild to counteract water bloom in China. However, little genomic information is available for this species, especially regarding its ability to grow rapidly in water, even water contaminated with high concentrations of poisonous microcystin. In this study, we performed de novo transcriptome assembly and analysis of the 17.10 million short-read sequences produced by the Illumina paired-end sequencing technology. Using an improved multiple k-mer contig assembly method coupled with further scaffolding, 85,759 sequences were obtained. There were 23,044 sequences annotated with 3423 gene ontology terms for 104 196 term occurrences and the three corresponding organizing princ

SUBMITTER: Fu B 

PROVIDER: S-EPMC3325077 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature