The mechanism of the translocation step in DNA replication by DNA polymerase I: a computer simulation analysis.

ABSTRACT: High-fidelity DNA polymerases copy DNA rapidly and accurately by adding correct deoxynucleotide triphosphates to a growing primer strand of DNA. Following nucleotide incorporation, a series of conformational changes translocate the DNA substrate by one base pair step, readying the polymerase for the next round of incorporation. Molecular dynamics simulations indicate that the translocation consists globally of a polymerase fingers-opening transition, followed by the DNA displacement and the insertion of the template base into the preinsertion site. They also show that the pyrophosphate release facilitates the opening transition and that the universally conserved Y714 plays a key role in coupling polymerase opening to DNA translocation. The transition involves several metastable intermediat

SUBMITTER: Golosov AA 

PROVIDER: S-EPMC3325112 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature