Intracellular Streptococcus pyogenes in human macrophages display an altered gene expression profile.

ABSTRACT: Streptococcus pyogenes is an important human pathogen, which has recently gained recognition as an intracellular microorganism during the course of severe invasive infections such as necrotizing fasciitis. Although the surface anchored M protein has been identified as a pivotal factor affecting phagosomal maturation and S. pyogenes survival within macrophages, the overall transcriptional profile required for the pathogen to adapt and persist intracellularly is as of yet unknown. To address this, the gene expression profile of S. pyogenes within human macrophages was determined and compared to that of extracellular bacteria using customized microarrays and real-time qRT-PCR. In order to model the early phase of infection involving adaptation to the intracellular compartment, samples were co

SUBMITTER: Hertzen E 

PROVIDER: S-EPMC3325220 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature