A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions.

ABSTRACT: Low-affinity extracellular protein interactions are critical for cellular recognition processes, but existing methods to detect them are limited in scale, making genome-wide interaction screens technically challenging. To address this, we report here the miniaturization of the AVEXIS (avidity-based extracellular interaction screen) assay by using protein microarray technology. To achieve this, we have developed protein tags and sample preparation methods that enable the parallel purification of hundreds of recombinant proteins expressed in mammalian cells. We benchmarked the protein microarray-based assay against a set of known quantified receptor-ligand pairs and show that it is sensitive enough to detect even very weak interactions that are typical of this class of interactions. The incr

SUBMITTER: Sun Y 

PROVIDER: S-EPMC3325482 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature