Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.

ABSTRACT:

Aim

To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.

Methods

Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.

Results

The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae,

SUBMITTER: Gustafsson RJ 

PROVIDER: S-EPMC3325529 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature