The clinical and environmental determinants of airway transcriptional profiles in allergic asthma.

ABSTRACT:

Rationale

Gene expression profiling of airway epithelial and inflammatory cells can be used to identify genes involved in environmental asthma.

Methods

Airway epithelia and inflammatory cells were obtained via bronchial brush and bronchoalveolar lavage (BAL) from 39 subjects comprising three phenotypic groups (nonatopic nonasthmatic, atopic nonasthmatic, and atopic asthmatic) 4 hours after instillation of LPS, house dust mite antigen, and saline in three distinct subsegmental bronchi. RNA transcript levels were assessed using whole genome microarrays.

Measurements and main results

Baseline (saline exposure) differences in gene expression were related to airflow obstruction in epithelial cells (C3, ALOX5AP, CCL18, and others), and to serum IgE (innate immune genes and

SUBMITTER: Yang IV 

PROVIDER: S-EPMC3326287 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature