HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells.

ABSTRACT: Epigenetic regulation of gene expression is important in maintaining self-renewal of embryonic stem (ES) and trophoblast stem (TS) cells. Histone deacetylases (HDACs) negatively control histone acetylation by removing covalent acetylation marks from histone tails. Because histone acetylation is a known mark for active transcription, HDACs presumably associate with inactive genes. Here, we used genome-wide chromatin immunoprecipitation to investigate targets of HDAC1 in ES and TS cells. Through evaluation of genes associated with acetylated histone H3 marks, and global expression analysis of Hdac1 knockout ES and trichostatin A-treated ES and TS cells, we found that HDAC1 occupies mainly active genes, including important regulators of ES and TS cells self-renewal. We also observed occupancy

SUBMITTER: Kidder BL 

PROVIDER: S-EPMC3326306 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature