Genetic screening of new genes responsible for cellular adaptation to hypoxia using a genome-wide shRNA library.

ABSTRACT: Oxygen is a vital requirement for multi-cellular organisms to generate energy and cells have developed multiple compensatory mechanisms to adapt to stressful hypoxic conditions. Such adaptive mechanisms are intricately interconnected with other signaling pathways that regulate cellular functions such as cell growth. However, our understanding of the overall system governing the cellular response to the availability of oxygen remains limited. To identify new genes involved in the response to hypoxic stress, we have performed a genome-wide gene knockdown analysis in human lung carcinoma PC8 cells using an shRNA library carried by a lentiviral vector. The knockdown analysis was performed under both normoxic and hypoxic conditions to identify shRNA sequences enriched or lost in the resulting s

SUBMITTER: Yoshino S 

PROVIDER: S-EPMC3327663 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature