Comprehensive copy number and gene expression profiling of the 17q23 amplicon in human breast cancer.

ABSTRACT: The biological significance of DNA amplification in cancer is thought to be due to the selection of increased expression of a single or few important genes. However, systematic surveys of the copy number and expression of all genes within an amplified region of the genome have not been performed. Here we have used a combination of molecular, genomic, and microarray technologies to identify target genes for 17q23, a common region of amplification in breast cancers with poor prognosis. Construction of a 4-Mb genomic contig made it possible to define two common regions of amplification in breast cancer cell lines. Analysis of 184 primary breast tumors by fluorescence in situ hybridization on tissue microarrays validated these results with the highest amplification frequency (12.5%) observed f

SUBMITTER: Monni O 

PROVIDER: S-EPMC33278 | biostudies-literature | 2001 May

REPOSITORIES: biostudies-literature