Mucin gene expression and mouse middle ear epithelium.

ABSTRACT:

Objectives

To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).

Methods

MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.

Results

Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.

Conclusion

Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expresse

SUBMITTER: Kerschner JE 

PROVIDER: S-EPMC3327946 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature