Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep.

ABSTRACT:

Background

A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, the role of LTR in tropism was unclear and env encoded sequences, likely involved in tropism, were not investigated. This study aimed to analyze heterogeneity of SRLV Env regions--TM amino terminal and SU V4, C4 and V5 segments--in order to assess virus compartmentalization in CNS.

Results

Eight Visna (neurologically) affected sheep of the outbreak were used. Of the 350 clones obtained after PCR amplification, 142 corresponded to CNS samples (spinal cord and choroid plexus) and the remaining to mammary gland, blood cells, bronchoalv

SUBMITTER: Ramirez H 

PROVIDER: S-EPMC3328241 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature