Nonlinear sorting, curvature generation, and crowding of endophilin N-BAR on tubular membranes.

ABSTRACT: The curvature of biological membranes is controlled by membrane-bound proteins. For example, during endocytosis, the sorting of membrane components, vesicle budding, and fission from the plasma membrane are mediated by adaptor and accessory proteins. Endophilin is a peripherally binding membrane protein that functions as an endocytic accessory protein. Endophilin's membrane tubulation capacity is well known. However, to understand the thermodynamic and mechanical aspects of endophilin function, experimental measurements need to be compared to quantitative theoretical models. We present measurements of curvature sorting and curvature generation of the endophilin A1 N-BAR domain on tubular membranes pulled from giant unilamellar vesicles. At low concentration, endophilin functions primarily

SUBMITTER: Zhu C 

PROVIDER: S-EPMC3328703 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature