RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls.

ABSTRACT:

Background

RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients.

Methods

Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9) and normal controls (N = 7) were analyzed by microarray analysis. Samples were collected according to protocols approved by the Institutional Review Board. Differential expressions were validated by qRT-PCR in a separate set of samples (N = 10 in both groups).

Results

Expression profiles of microvesicle RNA correctly separated individuals in two groups by unsupervised clustering. The most significant differences pertained to down-regulated genes (121 ge

SUBMITTER: Noerholm M 

PROVIDER: S-EPMC3329625 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature