Redesigning the NEDD8 pathway with a bacterial genetic screen for ubiquitin-like molecule transfer.

ABSTRACT: Pathways of ubiquitin-like (UBL) molecule transfer regulate a myriad of cellular cascades. Here, we report a high-throughput assay that correlates catalytic human NEDD8 transfer to bacterial survival. The assay was utilized to screen mutant NEDD8 and NAE (NEDD8-activating enzyme) libraries to engineer a more stable NEDD8 and redesign the NEDD8-NAE interface. This approach will be useful in understanding the specificities underlying UBL pathways.

SUBMITTER: Guntas G 

PROVIDER: S-EPMC3329750 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature