A cell-based phenotypic assay to identify cardioprotective agents.

ABSTRACT:

Rationale

Tissue ischemia/reperfusion (IR) injury underlies several leading causes of death such as heart-attack and stroke. The lack of clinical therapies for IR injury may be partly due to the difficulty of adapting IR injury models to high-throughput screening (HTS).

Objective

To develop a model of IR injury that is both physiologically relevant and amenable to HTS.

Methods and results

A microplate-based respirometry apparatus was used. Controlling gas flow in the plate head space, coupled with the instrument's mechanical systems, yielded a 24-well model of IR injury in which H9c2 cardiomyocytes were transiently trapped in a small volume, rendering them ischemic. After initial validation with known protective molecules, the model was used to screen a 2000-molecule

SUBMITTER: Guo S 

PROVIDER: S-EPMC3329894 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature