Spindle position in symmetric cell divisions during epiboly is controlled by opposing and dynamic apicobasal forces.

ABSTRACT: Orientation of cell division is a vital aspect of tissue morphogenesis and growth. Asymmetric divisions generate cell fate diversity and epithelial stratification, whereas symmetric divisions contribute to tissue growth, spreading, and elongation. Here, we describe a mechanism for positioning the spindle in symmetric cell divisions of an embryonic epithelium. We show that during the early stages of epiboly, spindles in the epithelium display dynamic behavior within the plane of the epithelium but are kept firmly within this plane to give a symmetric division. This dynamic stability relies on balancing counteracting forces: an apically directed force exerted by F-actin/myosin-2 via active cortical flow and a basally directed force mediated by microtubules and myosin-10. When both forces are

SUBMITTER: Woolner S 

PROVIDER: S-EPMC3332010 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature