Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats.

ABSTRACT: Arthritis susceptibility genes were sought by analysis of differential gene expression between pristane-induced arthritis (PIA)-susceptible DA rats and PIA-resistant E3 rats. Inguinal lymph nodes of naïve animals and animals 8 days after pristane injection were analyzed for differential gene expression. mRNA expression was investigated by microarray and real-time PCR, and protein expression was analyzed by flow cytometry or ELISA. Twelve genes were significantly differentially expressed when analyzed by at least two independent methods, and an additional five genes showed a strong a tendency toward differential expression. In naïve DA rats IgE, the bone marrow stromal cell antigen 1 (Bst1) and the MHC class II beta-chain (MhcII) were expressed at a higher level, and the immunoglobulin kapp

SUBMITTER: Wester L 

PROVIDER: S-EPMC333422 | biostudies-literature | 2003

REPOSITORIES: biostudies-literature