Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells.

ABSTRACT:

Background

During the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have been restricted to whole embryos. Here we report the first such analysis for primordial germ cells (PGCs), the progenitors of the germ-line stem cells.

Results

We purified PGCs from Drosophila embryos, defined their proteome and transcriptome, and assessed the content, scale and dynamics of their MZT. Transcripts encoding proteins that implement particular types of biological functions group into nine distinct expression profiles, reflecting coordinate control at the transcriptional a

SUBMITTER: Siddiqui NU 

PROVIDER: S-EPMC3334568 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature