Visualization and curve-parameter estimation strategies for efficient exploration of phenotype microarray kinetics.

ABSTRACT:

Background

The Phenotype MicroArray (OmniLog® PM) system is able to simultaneously capture a large number of phenotypes by recording an organism's respiration over time on distinct substrates. This technique targets the object of natural selection itself, the phenotype, whereas previously addressed '-omics' techniques merely study components that finally contribute to it. The recording of respiration over time, however, adds a longitudinal dimension to the data. To optimally exploit this information, it must be extracted from the shapes of the recorded curves and displayed in analogy to conventional growth curves.

Methodology

The free software environment R was explored for both visualizing and fitting of PM respiration curves. Approaches using either a model fit (and common

SUBMITTER: Vaas LA 

PROVIDER: S-EPMC3334903 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature