Retinal gene expression changes related to IOP exposure and axonal loss in DBA/2J mice.

ABSTRACT:

Purpose

To determine the effects of cumulative IOP exposure and axonal damage on retinal gene expression in DBA/2 mice.

Methods

DBA/2J, DBA/2J(pe) (pearl), and C57BL/6 mice from 3 to 12 months of age were used. IOP was measured with a rebound tonometer, and optic nerve (ON) damage was determined by grading of ON sections. Retinal RNA was subjected to microarray analysis. Comparisons explored the effects of cumulative IOP exposure (cIOPx) as well as ON damage (ONd) in the DBA/2J animals compared with that in the C57BL/6 and pearl mice. RT-PCR was performed to confirm some of the genes and bioinformatic analysis to identify affected gene networks.

Results

Microarrays revealed that an increasing number of genes were up- or downregulated in 9- and 12-month DBA/2J mice wit

SUBMITTER: Panagis L 

PROVIDER: S-EPMC3335131 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature