Transcriptome of protoplasts reprogrammed into stem cells in Physcomitrella patens.

ABSTRACT:

Background

Differentiated plant cells can retain the capacity to be reprogrammed into pluripotent stem cells during regeneration. This capacity is associated with both cell cycle reactivation and acquisition of specific cellular characters. However, the molecular mechanisms underlying the reprogramming of protoplasts into stem cells remain largely unknown. Protoplasts of the moss Physcomitrella patens easily regenerate into protonema and therefore provide an ideal system to explore how differentiated cells can be reprogrammed to produce stem cells.

Principal findings

We obtained genome-wide digital gene expression tag profiles within the first three days of P. patens protoplast reprogramming. At four time-points during protoplast reprogramming, the transcript levels of 4827

SUBMITTER: Xiao L 

PROVIDER: S-EPMC3335808 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature