CENP-C facilitates the recruitment of M18BP1 to centromeric chromatin.

ABSTRACT: Centromeres are important structural constituents of chromosomes that ensure proper chromosome segregation during mitosis by providing defined sites for kinetochore attachment. In higher eukaryotes, centromeres have no specific DNA sequence and thus, they are rather determined through epigenetic mechanisms. A fundamental process in centromere establishment is the incorporation of the histone variant CENP-A into centromeric chromatin, which provides a binding platform for the other centromeric proteins. The Mis18 complex, and, in particular, its member M18BP1 was shown to be essential for both incorporation and maintenance of CENP-A. Here we show that M18BP1 displays a cell cycle-regulated association with centromeric chromatin in mouse embryonic stem cells. M18BP1 is highly enriched at cen

SUBMITTER: Dambacher S 

PROVIDER: S-EPMC3337164 | biostudies-literature | 2012 Jan-Feb

REPOSITORIES: biostudies-literature