Does singlet oxygen activate cell death in Arabidopsis cell suspension cultures?: analysis of the early transcriptional defense responses to high light stress.

ABSTRACT: Can Arabidopsis cell suspension cultures (ACSC) provide a useful working model to investigate genetically-controlled defense responses with signaling cascades starting in chloroplasts? In order to provide a convincing answer, we analyzed the early transcriptional profile of Arabidopsis cells at high light (HL). The results showed that ACSC respond to HL in a manner that resembles the singlet oxygen ((1)O(2))-mediated defense responses described for the conditional fluorescent (flu) mutant of Arabidopsis thaliana. The flu mutant is characterized by the accumulation of free protochlorophyllide (Pchlide) in plastids when put into darkness and the subsequent production of (1)O(2) when the light is on. In ACSC, (1)O(2) is produced in chloroplasts at HL when excess excitation energy flows into p

SUBMITTER: Gutierrez J 

PROVIDER: S-EPMC3337182 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature