Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association.

ABSTRACT:

Background

The genome of Mycobacterium avium subspecies paratuberculosis (MAP) is remarkably homogeneous among the genomes of bovine, human and wildlife isolates. However, previous work in our laboratories with the bovine K-10 strain has revealed substantial differences compared to sheep isolates. To systematically characterize all genomic differences that may be associated with the specific hosts, we sequenced the genomes of three U.S. sheep isolates and also obtained an optical map.

Results

Our analysis of one of the isolates, MAP S397, revealed a genome 4.8 Mb in size with 4,700 open reading frames (ORFs). Comparative analysis of the MAP S397 isolate showed it acquired approximately 10 large sequence regions that are shared with the human M. avium subsp. hominissuis strai

SUBMITTER: Bannantine JP 

PROVIDER: S-EPMC3337245 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature